Fiche détaillée de la technologie

 
Titre de la technologie / Technology Title
    Procédé pour améliorer la résolution en microscopie optique
Description / Description
    A method and a system for obtaining a high-resolution image of a volume of a sample using laser imaging are provided. The method includes a step of probing the volume of the sample with a first excitation beam having an intensity profile of maximum intensity at a center thereof, thereby obtaining a positive image of the volume. The method also in cludes a step of probing the volume of the sample with a second excitation beam having an intensity profile of minimum intensity at a center thereof and defining a peripheral region of maximum intensity around the center, thereby obtaining a negative image of the volume. The method finally includes a step of subtracting the negative image from the positive image, thereby obtaining the high- resolution image of the volume of the sample. Advantageously, embodiments of the invention can be probe- and fluorescence-independent, and be conveniently retrofitted into existing laser imaging systems.
Inventeur / Inventor Collaborateur(s) / Collaborator(s) Contact / Contact Champs(s) d'intérêt(s) / Industry segment(s) Brevet(s) / Patent(s) Numéro de la technologie / File Number : 01249
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